| Sat 06-Aug-2016 |
|---|
| 15:00 | Edgware Town | 5‑1 | Holmer Green | |
| 15:00 | Ely City | 1‑4 | Deeping Rangers | |
| 15:00 | Enfield FC | 2‑4 | Tower Hamlets | |
| 15:00 | Erith & Belvedere | 0‑1 | Loxwood | |
| 15:00 | Erith Town | 2‑2 | Sutton Common Rovers | |
| 15:00 | Eynesbury Rovers | 3‑1 | Peterborough Northern Star | |
| 15:00 | Fairford Town | 1‑5 | CB Hounslow United FC | |
| 15:00 | Fakenham Town | 0‑1 | Sleaford Town | |
| 15:00 | FC Broxbourne Borough | 1‑5 | Felixstowe & Walton | |
| 15:00 | FC Clacton | 3‑5 | Eton Manor | |
| 15:00 | Flackwell Heath | 6‑3 | Baldock Town | |
| 15:00 | Godmanchester Rovers | 2‑0 | Great Yarmouth Town | |
| 15:00 | Hailsham Town | 5‑7 | Crawley Down Gatwick | |
| 15:00 | Hanworth Villa | 2‑2 | Knaphill | |
| 15:00 | Harefield United | 1‑1 | Northampton On Chenecks | |
